Identifies the complete range of Enterobacteriaceae and other non-fastidious, oxidase positive and negative, gram negative bacilli. The GN-A kit includes 60 microwell strips in foil pouches, frame for microwell strips, result forms, instructions for use and color chart for reading results. GN-A kit can be used separately from GN-B kit. GN-B kit includes 24 microwell strips in foil pouches and instructions for use. GN-B kit id designed to be used together with GN-A kit and not on its own. Identification software and reagents must be purchased separately.
The GN-ID system comprises two separate, 12-substrate, microwell strips; the GN-A microwell strip and the GN-B microwell strip. The GN-A strip can be used separately and identifies the most commonly encountered, oxidase negative, non-fastidious, gram negative bacilli. GN-B is designed to be used in conjunction with GN-A; the combined strips identify the complete range of Enterobacteriaceae and other non-fastidious, oxidase positive and negative, gram negative bacilli. The wells in each microwell strip contain dehydrated biochemical substrates that are reconstituted with a saline suspension of the organism to be identified. If the substrate found in each well is metabolized by the organism, then a color change occurs during incubation or after the addition of specific reagents. Substrates are based on conventional biochemical methods used for identifying Enterobacteriaceae and other gram negative bacilli.
A pure, 18-24 hour culture of the isolate to be identified must always be used. We suggest selecting a single colony from EZ-CHROM chromogenic media. An oxidase test must be performed on the isolate prior to strip inoculation. Oxidase positive organisms can only be identified when A and B strips are both inoculated. Emulsify a single colony from an 18-24 hour culture in sterile physiological saline (3 ml for strip A, 3-5 mls for A+B). Mix thoroughly. Add 3-4 drops (100µL) of the suspension to each well of the strip(s). After inoculation, overlay specified wells with 3-4 drops of mineral oil; these wells are highlighted with a black circle around the well to assist in adding oil to the correct wells. Seal the top of the microwell strip(s) with adhesive strip provided and incubate at 35-37oC for 18-24 hours. Remove adhesive strip and record all positive reaction with the aid of the color chart. Add Indole Kovacs, VP I & II, Nitrate A & B and TDA reagents to the appropriate wells as described in the Instructions for Use. Read and record results using the color chart and Identification Software (sold separately). For a complete explanation of procedure for use, please refer to the Instructions for Use, included with your kit.
Gram Negative (GN) ID | |
Storage |
The microwell strips are stable in the unopened foil pouches at 2-8oC until the expiry date stated. Once the foil pouch has been opened, unused microwell strips should be replaced into the pouch and the pouch resealed. Microwell strips in resealed pouches should be used within 14 days. |
Shelf Life | 1 year |
Well Substrates | |
GN-A Strip |
Well 1- Lysine Well 2- Ornithine Well 3- H2S Well 4- Gluclose Well 5- Mannitol Well 6- Xylose Well 7- ONPG Well 8- Indole Well 9- Urease Well 10- VP Well 11- Citrate Well 12- TDA |
GN-B Strip |
Well 13- Gelatin Well 14- Malonate Well 15- Inositol Well 16- Sorbitol Well 17- Rhamnose Well 18- Sucrose Well 19- Lactose Well 20- Arabinose Well 21- Adonitol Well 22- Raffinose Well 23- Salicin Well 24- Arginine |
Reagents |
Indole Kovacs TDA Voges-Proskauer I Voges-Proskauer II Nitrate Reagent A Nitrate Reagent B Mineral Oil |