Salmonella Latex Agglutination Kit



A latex agglutination presumptive test for the presence of Listeria spp to confirm colonies on agar plate media. Microgen Listeria latex may be used in conjunction with biochemical tests (Microgen Listeria-ID) for further species identification.

Listeria Latex Brochure

TN12-15

Benefits

  • Results in 2 minutes
  • Cost effective
  • High sensitivity
  • High specificity
  • Accurate & reliable results
  • User friendly procedure

Detects the Listeria genus, including these species:

  • Listeria monocytogenes
  • Listeria innocua
  • Listeria welshimeri
  • Listeria ivanovii
  • Listeria seeligeri
  • Listeria grayi

Included in the kit:

  • 2.5mls Test latex
  • 0.5ml Positive control
  • 5mls Isotonic Saline
  • disposable reaction cards
  • disposable mixing sticks

Method from plates

  1. Place 1 drop of saline on to a well of the reaction card
  2. Remove a typical looking colony from the plate using a loop and emulsify in the drop of saline
  3. Rock the slide gently for 2mins to check for auto agglutination
  4. Add 1 drop of test latex to the suspension, mix using a mixing stick
  5. Rock the slide for up to 2 minutes and examine for agglutination

Interpretation

  • Agglutination within 2 minutes is indicative of the presence of Listeria spp in the sample
  • Absence of agglutination indicates that Listeria spp are not present in the sample

Latex Agglutination Principle

  • Polyvalent antisera raised against the 4 Listeria flagella antigens is used to coat latex particles.
  • When mixed with a suspension of Listeria containing these antigens, the latex particles rapidly agglutinate forming visible clumps

Latex technology:

  • Producer strains carrying specific antigens are cultured at optimal temperature for flagella production
  • isolates sub cultured on to Jameson plates to select most motile organisms
  • strains cultivated in broth
  • Supernatant discarded and cell deposit treated to harvest flagella
  • flagella immunogen used to generate antisera
  • antisera titres checked and blended
  • blended antisera purified and then coated on latex particles

Listeria spp possess 4 major flagellar antigen types designated A, B, C and E. AllListeria spp possess one or more of A, B, or C butL. grayi only possess E. Antiserum used to coat the latex particles must contain all 4 flagella antigens in order to detect all 6 Listeria spp.

Technical Information for Listeria Latex Agglutination Kit
Storage: Store at 2-8°C
Shelf Life: 1 year
Detects the Listeria genus,
including these species:
  • Listeria monocytogenes
  • Listeria innocua
  • Listeria welshimeri
  • Listeria ivanovii
  • Listeria seeligeri
  • Listeria grayi